High-Resolution Imaging

Keyser
Kent Keyser, Ph.D. – Director

Mission

This Shared Facility’s mission is to provide state-of-the-art imaging resources and the technical support to use them effectively for Cancer Center investigators as well as other investigators in the UAB research community.

Facility Description

The High Resolution Imaging Facility or “HRIF” is a cutting-edge facility providing a variety of microscopy services for UAB Scientists.  Investigators conducting experiments that require the use of high end digital microscopes and technical expertise can benefit from the facility’s diverse resources.

Research Information

Fluorescence Microscopy, Confocal Laser Microscopy, Time Lapse, FRET, FRAP, Electron Microscopy, Cryo-EM Microscopy and Light Microscopy are available at the “HRIF.”  Consultation and training are available for all users of the facility by a staff of highly trained professionals.

Services and Fees

Surcharge for Assistance in Instrumentation or Sample Preparation
$15.00/Hour

Basic Digital Imaging/Deconvolution/ Calcium Imaging — Olympus IX70
$15.00/Hour

Basic Digital Imaging –Leitz Orthoplan, Nikon Diaphot
$15.00/Hour

Confocal Laser Scanning Microscopy – Leica SP1
$26.00/Hour

Confocal Laser Scanning Microscopy with UV Laser – Leica
$26.00/Hour

Confocal Laser Scanning Microscopy — Olympus Fluoview
$26.00/Hour

Digital Imaging/Timelapse/FRET/Histology Imaging – Nikon Eclipse
$20.00/Hour

FRET/FRAP Confocal Laser Scanning Microscopy – Leica SP2
$26.00/Hour

Large Specimen/Tissue Dissection Imaging Nikon Stereoscope
$20.00/Hour

Data Archival Service Fee (includes blank CD)
$3.00 each

High Resolution Printing: Inkjet
$3.00 each

Transmission Electron Microscopy— FEI Tecnai T12 Spirit/Assisted
$60.00/Hour

Transmission Electron Microscopy— FEI Tecnai T12 Spirit/Unassisted
$30.00/Hour

Sample processing per sample/Bulk Discounts Available
$75.00/Sample

Ultra-thin and semi-thin sectioning per sample with post stain
$40.00/Hour

Negative Staining per Grid
$10.00/Grid

Cryo-EM Usage Technai F20 Full Service Access
$25/Hour

Cryo-EM Usage Technai F20 Full Service Access
$25/Hour

Cryo-EM Usage Technai F20  Reduced Service Access
$15/Hour

Cryo-EM Grid for Sample Preparation
$10.00/Grid

Contact Information:

Director:  Kent Keyser, Ph.D.

Email:  ktkeyser@uab.edu

Phone: 975-7225

Technical Staff:

Shawn Williams – Microscopist, Digital Imaging Specialist, Confocal Microscopy Specialist

Email:  tzaron@uab.edu

Phone:  934-7403

Ed Phillips – General Microscopy and Sample Preparation, Fluorescence, Histology, Electron Microscopy

Email:  dogfan@uab.edu

Phone:  934-7855

For questions pertaining to Electron Microscopy and EM Sample Preparation, please contact:

Melissa Chimento – Electron Microscopist – Transmission EM, Sample Preparation

Email:  mchimento@uab.edu

Phone:  934-1926

Terje Dokland, Ph.D. – Cryo-Electron Microscopist, Cryo-EM Sample Preparation

Email:  dokland@uab.edu

Phone:  996-4502 or 996-4510

Website:  http://main.uab.edu/show.asp?durki=34926

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