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Kent Keyser, Ph.D. - Director
Mission
This Shared Facility’s mission is to provide state-of-the-art imaging resources and the technical support to use them effectively for Cancer Center investigators as well as other investigators in the UAB research community.
Facility Description
The High Resolution Imaging Facility or “HRIF” is a cutting-edge facility providing a variety of microscopy services for UAB Scientists. Investigators conducting experiments that require the use of high end digital microscopes and technical expertise can benefit from the facility’s diverse resources.
Research Information
Fluorescence Microscopy, Confocal Laser Microscopy, Time Lapse, FRET, FRAP, Electron Microscopy, Cryo-EM Microscopy and Light Microscopy are available at the “HRIF.” Consultation and training are available for all users of the facility by a staff of highly trained professionals.
Services and Fees
Surcharge for Assistance in Instrumentation or Sample Preparation
$15.00/Hour
Basic Digital Imaging/Deconvolution/ Calcium Imaging -- Olympus IX70
$15.00/Hour
Basic Digital Imaging –Leitz Orthoplan, Nikon Diaphot
$15.00/Hour
Confocal Laser Scanning Microscopy – Leica SP1
$26.00/Hour
Confocal Laser Scanning Microscopy with UV Laser – Leica
$26.00/Hour
Confocal Laser Scanning Microscopy -- Olympus Fluoview
$26.00/Hour
Digital Imaging/Timelapse/FRET/Histology Imaging – Nikon Eclipse
$20.00/Hour
FRET/FRAP Confocal Laser Scanning Microscopy – Leica SP2
$26.00/Hour
Large Specimen/Tissue Dissection Imaging Nikon Stereoscope
$20.00/Hour
Data Archival Service Fee (includes blank CD)
$3.00 each
High Resolution Printing: Inkjet
$3.00 each
Transmission Electron Microscopy--- FEI Tecnai T12 Spirit/Assisted
$60.00/Hour
Transmission Electron Microscopy--- FEI Tecnai T12 Spirit/Unassisted
$30.00/Hour
Sample processing per sample/Bulk Discounts Available
$75.00/Sample
Ultra-thin and semi-thin sectioning per sample with post stain
$40.00/Hour
Negative Staining per Grid
$10.00/Grid
Cryo-EM Usage Technai F20 Full Service Access
$25/Hour
Cryo-EM Usage Technai F20 Full Service Access
$25/Hour
Cryo-EM Usage Technai F20 Reduced Service Access
$15/Hour
Cryo-EM Grid for Sample Preparation
$10.00/Grid
Contact Information:
Director: Kent Keyser, Ph.D.
Email:
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Phone: 975-7225
Technical Staff:
Shawn Williams - Microscopist, Digital Imaging Specialist, Confocal Microscopy Specialist
Email:
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Phone: 934-7403
Ed Phillips - General Microscopy and Sample Preparation, Fluorescence, Histology, Electron Microscopy
Email:
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Phone: 934-7855
For questions pertaining to Electron Microscopy and EM Sample Preparation, please contact:
Melissa Chimento - Electron Microscopist - Transmission EM, Sample Preparation
Email:
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Phone: 934-1926
Terje Dokland, Ph.D. - Cryo-Electron Microscopist, Cryo-EM Sample Preparation
Email:
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Phone: 996-4502 or 996-4510
Website: http://main.uab.edu/show.asp?durki=34926
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